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Thermoresponsive Oligo(ethylene glycol)-Based Polymer Brushes on Polymer Monoliths for All-Aqueous Chromatography
journal contribution
posted on 2013-12-11, 00:00 authored by Nan Li, Li Qi, Ying Shen, Yaping Li, Yi ChenPorous polymer monoliths
onto which were grafted a thermoresponsive
copolymer, poly(2-(2-methoxyethoxy)ethyl methacrylate (MEO2MA)-co-oligo(ethylene glycol) methacrylate (OEGMA)),
were synthesized by the two-step atom transfer radical polymerization
(ATRP) method. The copolymer-grafted monoliths were characterized
by elemental analysis, scanning electron microscopy, and mercury intrusion
porosimetry. They were further used as the thermoresponsive stationary
phase for all-aqueous high-performance liquid chromatography (HPLC).
The chromatograms of three steroids demonstrated that the chain length
of the grafted copolymer, which was regulated by varying the grafting
time, could affect the separation by providing different amounts of
hydrophobic interaction sites with analytes. Additionally, the elution
profiles of steroids on the stationary phase could also be tuned by
the comonomer composition. The results showed that the porous polymer
monoliths enabled separation of the test mixture in pure aqueous mobile
phase under isocratic conditions. Furthermore, the proposed method
provides a simple and promising tool in the design and construction
of responsive surfaces for chromatography applications.
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polymer monolithsthermoresponsive copolymerOEGMAMEO 2MA glycolchain lengthchromatography applicationsHPLCmethodinteraction siteselution profilescomonomer compositionATRPscanning electron microscopyseparationphaseisocratic conditionsPolymer Monolithsatom transfersteroidtest mixturemercury intrusion porosimetrymethacrylate
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