The role of the Psc1 RS domain in Psc1 subcellular localization

Copyright information:

Taken from "A family of RS domain proteins with novel subcellular localization and trafficking"

Nucleic Acids Research 2005;33(4):1309-1322.

Published online 1 Mar 2005

PMCID:PMC552957.

© The Author 2005. Published by Oxford University Press. All rights reserved

() Subcellular distribution of Psc1 protein in Psc1, GFP–Psc1ΔRS and GFP–RS transfected COS-1 cells. Error bars indicate standard deviation. () COS-1 cell transfected with GFP–Psc1ΔRS and visualized by direct fluorescence (lower right). Nuclei stained with Hoechst 333258 (lower left). The top panel shows merged images. () COS-1 cell transfected with GFP–Psc1ΔRS and visualized by direct fluorescence (lower right) or anti-SC35 antibody (lower left). Top panel shows merged images. () COS-1 cell co-transfected with GFP–Psc1ΔRS and Psc1-HA, and visualized by direct fluorescence (lower right) or anti-HA TRITC (lower left). Top panel shows merged images. () COS-1 cell transfected with GFP–RS and visualized by direct fluorescence (lower right) or anti SC35 antibody (lower left). Top panel shows merged images. () COS-1 cell transfected with GFP–RS and visualized by direct fluorescence showing diffuse distribution in the nucleus and the cytoplasm as observed in 30% of cells. All images were captured using conventional microscopy. Size bars represent 10 μm.