The role of C-terminal elements in Psc1 subcellular localization

Copyright information:

Taken from "A family of RS domain proteins with novel subcellular localization and trafficking"

Nucleic Acids Research 2005;33(4):1309-1322.

Published online 1 Mar 2005

PMCID:PMC552957.

© The Author 2005. Published by Oxford University Press. All rights reserved

() Subcellular distribution of Psc1 protein in Psc1, GFP–Psc1ΔCD and GFP–CD transfected COS-1 cells. Error bars indicate standard deviation. () COS-1 cell cotransfected with GFP–Psc1ΔCD and Psc1–HA, visualized by direct fluorescence (lower right) or anti-HA TRITC (lower left). Top panel shows merged images. The nucleus is outlined by a dotted line. () COS-1 cell transfected with GFP–Psc1ΔCD, visualized by direct fluorescence (lower right) or anti-SC35 antibody (lower left). GFP–Psc1ΔCD is restricted to the nucleus in this example. Top panel shows merged images. () Representative cytoplasmic section of COS-1 cells transfected with GFP–CD and visualized by anti α-tubulin antibody (i) or by direct fluorescence (ii). Merged images shown in (iii). Size bars represent 10 μm. All images were obtained by conventional microscopy.