The expression of nestin and Sox2 without and with DC pulse stimulation.

The percentage of immunostained cells with (A) nestin and (B) Sox2 for mouse neural stem and progenitor cells (mNPCs) cultured with (electrical field [EF]) and without (control [CTL]) square-wave DC pulse stimulation. The P values of Sox2 expression at 3 and 14 DIV for the CTL and EF groups were 0.080 and 0.071, respectively.