The expression of Cdk5/p35 in the immortalized mouse podocytes and the cortexes of mouse kidneys over time.

A. Western blotting analysis showed the p35 and Cdk5 expression levels in the cultured mouse podocytes at 0 day (d0: lane1), 2 days (d2: lane 2), 4 days (d4: lane 3), 8 days (d8: lane 4) and 10 days (d10: lane 5). B. The bar graph illustrates an increasing Cdk5 activity in the cultured mouse podocytes from day 0 to day 10. C. Western blotting showed the p35 and Cdk5 expression levels in the cortexes from embryos to the adult: E14 (lane 1), E18 (lane 2), E22 (lane 3), p2 (lane 4) and adult (lane 5) mouse kidneys. D. bar graph showed an increasing Cdk5 activity levels in the cortexes of mouse kidneys during the above time (E14-adult). Data are expressed as means of three separate experiments.