The establishment of CVB3-induced VMC model.
4-week-old male C57BL/6 mice were injected intraperitoneally with 1000 TCID50 of CVB3 virus or an equal amount of DMEM on day 0. (A) Representative HE staining of paraffin sections of heart tissues from VMC mice and control mice on both day 7 and day 28 after virus injection. Scale bar: 50μm. (B) The pathologic score of myocarditis was assessed according to the mononuclear infiltration foci and myocardial necrosis. (C) Representative M-mode echocardiographic images of VMC mice and control mice on day 7 and day 28 post-infection. (D, E) Left ventricular ejection fraction (LVEF) and left ventricular fractional shortening (LVFS) from the echocardiographic data for both day 7 and day 28 post-infection. (Con: control group; CVB3: coxsackievirus B3 group; Con: n = 10; CVB3: n = 10; ***P<0.001 vs. Con-day 7; ##P<0.01 vs. Con-day 28; ###P<0.001 vs. Con-day 28).
