The effects of apoE genotype and CS-6253 on the levels and lipidation of brain apoA-I in apoE3 and apoE4 mice.

(A) Total apoA-I levels. ApoE3 and apoE4 mice were injected i.p. with CS-6253 or PBS, after which their hippocampi was extracted and immunoblotted utilizing anti-apoA-I Ab, as described in the Materials and Methods. Representative immunoblots of four mice per group are presented on the upper panel. Quantitation of the results (mean ± SEM; n = 5 per group) normalized relative to control apoE3 mice is presented on the bottom panel. White bars correspond to apoE3 mice, whereas black bars correspond to apoE4 mice. Two-way ANOVA revealed a significant effect for genotype (p = 0.003). *p<0.05 for the post hoc comparison of control apoE4 and control apoE3 mice, and *p<0.05 for the post hoc comparison of CS-6253-treated apoE4 and CS-6253 treated apoE3 mice. (B) Native apoA-I gels. Hippocampal extracts (n = 4 per group) from control and CS-6253 treated mice were electrophoresed on 4–16% gradient non-reducing native gel, as described in Materials and Methods. Representative immunoblots of three mice per group are presented and revealed that apoA-I migrated as two main bands of molecular highest of 25 and 45–50 kDa. The intensities of these native gel bands were not affected by CS-6253 and were higher in the apoE4 mice compared to the apoE3 mice.