pone.0174713.g003.tif (196.72 kB)
The absence of Mn2+ from the sporulation medium delays asymmetric Z-ring formation.
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posted on 2017-03-30, 18:06 authored by Ewa Cendrowicz, Anabela de Sousa Borges, Malgorzata Kopacz, Dirk-Jan ScheffersPie-chart representation of sporulating B. subtilis cells in the presence (+) and absence (-) of Mn2+. Four different types of cell (representative images in the legend) were scored: cells without any Z-ring in the cell (no ring), cells with a Z-ring in the middle of the rod (mid-cell ring), cells with two rings assembled at the cell poles (two polar rings) and cells with only one polar ring (polar ring). Non-sporulating cells are marked in blue shades, while sporulating cells are marked in orange shades. Each pie chart is the result of two independent classification experiments in which at least 290 cells were classified per condition. Actual percentages and standard deviations are included in S1 Table.
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light scattering assaylarger oligomeric structuresentire cytoplasmic partbacillus subtilis stabilizes ftsz polymersstimulates spoiie oligomerizationbifunctional protein involved2 + supsporulation medium resultsf supdiv >< pasymmetric septum formationspoiie requires mnspoiie phosphatase activityspoiie phosphatase activityspoiie proteinpolar ftszasymmetric divisionprotein copurifiesresults indicatenull mutantforespore compartmentbound metals
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