The RDV P2 protein interacts with OsIAA10.

(A) RDV P2 interacts with OsIAA10 in yeast. Yeast transformants were spotted on the control medium (SD-Leu/-Trp (SD-L-W)) and selection medium (SD-Leu/-Trp/-His/-Ade (SD-L-W-H-Ade)). AD, activating domain; BD, binding domain; SD, synthetic dropout. (B) Co-immunoprecipitation confirms the interaction between P2 and OsIAA10 in RDV-infected FLAG-OsIAA10 overexpressing (OsIAA10-OE) rice. WT, wild type rice; Mock, mock-inoculated rice; RDV, RDV-infected rice. (C) LCI assay shows interaction between P2 and OsIAA10 in vivo. The left diagram indicates the leaf panels that were infiltrated with A. tumefaciens containing the different combinations of indicated constructs. Cps indicates signal counts per second. (D) P2 interacts with domain II of OsIAA10. (E) Determination of the functional domains of P2 that interact with OsIAA10. The prey protein AD-OsIAA10 was expressed with the indicated bait proteins in yeast AH109 cells. Interaction was indicated by the ability of cells to grow on medium SD-L-W-H-Ade.