bc8b00596_si_002.mp4 (5.99 MB)
Templated Macroporous Polyethylene Glycol Hydrogels for Spheroid and Aggregate Cell Culture
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posted on 2018-12-18, 00:00 authored by Mozhdeh Imaninezhad, Lindsay Hill, Grant Kolar, Kyle Vogt, Silviya Petrova ZustiakMacroporous cell-laden
hydrogels have recently gained recognition
for a wide range of biomedical and bioengineering applications. There
are various approaches to create porosity in hydrogels, including
lyophilization or foam formation. However, many do not allow a precise
control over pore size or are not compatible with in situ cell encapsulation. Here, we developed novel templated macroporous
hydrogels by encapsulating uniform degradable hydrogel microspheres
produced via microfluidics into a hydrogel slab. The microspheres
degraded completely leaving macropores behind. Microsphere degradation
was dependent on the incubation medium, microsphere size, microsphere
confinement in the hydrogel as well as cell encapsulation. Uniquely,
the degradable microspheres were biocompatible and when laden with
cells, the cells were deposited in the macropores upon microsphere
degradation and formed multicellular aggregates. The hydrogel-encapsulated
cell aggregates were used in a small drug screen to demonstrate the
relevance of cell–matrix interactions for multicellular spheroid
drug responsiveness. Hydrogel-grown spheroid cultures are increasingly
important in applications such as in vitro tumor,
hepatocellular, and neurosphere cultures and drug screening; hence,
the templated cell aggregate-laden hydrogels described here would
find utility in various applications.
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Templated Macroporous Polyethylene Glycol Hydrogelscell encapsulationhydrogel-encapsulated cell aggregatesmulticellular spheroid drug responsivenesstemplated cell aggregate-laden hydrogelsapplicationencapsulating uniform degradable hydrogel microspheresnovel templated macroporous hydrogelsAggregate Cell Culture Macroporous cell-laden hydrogelsHydrogel-grown spheroid cultures
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