Fig 5.TIF (3.63 MB)
TUNEL staining of Capan-1 cells following a 48 h treatment with HMNE3.
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posted on 2016-10-19, 17:50 authored by Yong-Chao Ma, Zhi-Xin Wang, Shao-Ju Jin, Yan-Xin Zhang, Guo-Qiang Hu, Dong-Tao Cui, Jiang-Shuan Wang, Min Wang, Fu-Qing Wang, Zhi-Jun ZhaoLogarithmically growing cells were treated with the indicated doses of HMNE3 for 48 h, and the apoptotic cells were examined using the TUNEL assay. Cells from at least five randomly chosen areas on each slide were visualized and photographed using a fluorescent microscope. The percentage of apoptotic cells was determined by counting the number of apoptotic cells and dividing this value by the total number of cells in the areas.
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HMNE 3TUNELHRPtopoisomerase II activityreceptor tyrosine kinasetyrosine kinaseHMNE 3 dosestumor cell proliferationterminal deoxynucleotidyl transferasetopoisomerase II proteinsRTKDNA plasmid pBR 322agarose gel electrophoresisHoechst 33258 fluorescence staining3.2 μ M HMNE 3Human Pancreatic Cancer Cellsnovel bis-fluoroquinolone chalcone-liketopoisomerase IIIC 50 valuecancer cell linesNovel Bis-Fluoroquinolone Chalcone-Liketyrosine kinase activityMTT
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