TRAF-interacting-motifs are required for the interaction of MAVS with GP73.

(A) Scheme of TRAF6 protein and its mutants (top panel). HEK293 cells (2×10⁶) were co-transfected with GP73 (1 μg) and indicated plasmids (3 μg) for 24 h. Cell lysates were subjected to immunoprecipitation and WB with indicated antibodies. (B) Scheme of MAVS and its mutants. (C, D, E and F) Interactions of GP73 with MAVS and a series of truncations (C, D and E) or a series of TIM3 mutants (F). HEK293 cells (2×10⁶) were co-transfected with GP73 plasmid (1 μg) and indicated plasmids (3 μg) for 24 h. Cell lysates were subjected to immunoprecipitation and WB with indicated antibodies.