TGF-beta1 enhances the conversion of Treg cells in the presence of Lp eosinophils.

(A) MACS-sorted CD4+CD62L+ T cell from the OT-II mouse were cultured under activating coditions with LP or PB eosinophils as described in Figure 3 with IgG1 isotype control antibody (isotype) or TGF-β neutralizing antibody (a-TGF-β). The cells were stain for Foxp3 and CD4and analyzed by flow cytometry.

(B) RNA was isolated from purified LP or PB eosinophils, and expression of tgfb1 were analyzed by quantitative real-time PCR. Values are expressed relative to Gapdh. Bars show the means ± SD of three independent experiments.

(C) MACS-sorted CD4+CD62L+ T cell from the OT-II mouse were cultured under activating coditions with LP or PB eosinophils as described in Figure 3 with the indicated concentrations of TGF-β1. The cells were stain for Foxp3 and CD4and analyzed by flow cytometry.