Synthesis of a Polyhistidine-bearing Amphipol and its Use for Immobilizing Membrane Proteins
2015-12-14T00:00:00Z (GMT) by
Amphipols (APols) are short amphipathic polymers that stabilize membrane proteins (MPs) in aqueous solutions. In the present study, A8–35, a polyacrylate-based APol, was grafted with hexahistidine tags (His<sub>6</sub>-tags). The synthesis and characterization of this novel functionalized APol, named HistAPol, are described. Its ability to immobilize MPs on nickel ion-bearing surfaces was tested using two complementary methods, immobilized metal affinity chromatography (IMAC) and surface plasmon resonance (SPR). Compared to a single His<sub>6</sub>-tag fused at one extremity of a MP, the presence of several His<sub>6</sub>-tags carried by the APol belt surrounding the transmembrane domain of a MP increases remarkably the affinity of the protein/APol complex for nickel ion-bearing SPR chips, whereas it does not show such a strong effect on an IMAC resin. HistAPol-mediated immobilization, which allows reversibility of the interaction and easy regeneration of the supports and dispenses with any genetic modification of the target protein, provides a novel, promising tool for attaching MPs onto solid supports while stabilizing them.