Fig 2.TIF (466.32 kB)

Suppression of type I IFN induction by HCV F/ARFP.

figure

posted on 2016-07-12, 07:13 authored by Seung Bum Park, Scott Seronello, Wasima Mayer, David M. Ojcius

(A) Huh7 cells transfected with pEF (empty vector), pHA-F, pFLAG-NS3/4A, or pHA-F plus pFLAG-NS3/4A were analyzed by Western blots using anti-HA, FLAG, and actin antibodies. (B—D) Huh7 cells were transfected with indicated plasmids and, after 48 hrs, transfected with 5 μg of HCV RNA PAMP corresponding to the UTR or poly(IC). Samples were analyzed for the indicated mRNAs, 24 hrs after PAMP stimulation by qRT-PCR. For (B–D), star indicates statistically significant difference (P < 0.05) from respective minus PAMP controls. Letter “a” indicates statistically significant difference (P < 0.05) from the corresponding pEF control for each -PAMP or +PAMP group. Lines with P values also indicate statistically significant difference between those samples. All mRNA data were normalized by GAPDH mRNA and shown as percentage of controls.