Supplementary Material for: miR-155-5p is Negatively Associated with Acute Pancreatitis and Inversely Regulates Pancreatic Acinar Cell Progression by Targeting Rela and Traf3

<b><i>Background/Aims:</i></b> Acute pancreatitis contributes to high mortality in pancreatitis patients, and miRNAs play a vital role in the development of acute pancreatitis (AP), however, its precise biological role remains largely elusive. <b><i>Methods:</i></b> To clarify the potential mechanisms of miRNAs in AP, we built mouse models of mild acute pancreatitis (MAP) and moderate/ severe acute pancreatitis (SAP). MiRNA microarray analysis and Real-time quantitative PCR (qRT-PCR) were used to analyze the expression of miRNA in MAP/SAP. TargetScan software, dual-luciferase gene reporter assays and Western blotting were used to assess the target genes of miR-155-5p in AP. <b><i>Results:</i></b> miR-155-5p was significantly decreased in MAP/SAP mice compared to controls. In pancreatic acinar AR42J cells transfected with miR-155-5p mimic, the expression of <i>Rela</i> and <i>Traf3</i> notably decreased in both the caerulein- and TLC-S-induced groups compared with the negative control (NC); however, the expression of <i>Rela</i> and <i>Traf3</i> notably increased after transfection with miR-155-5p inhibitor. Combined analysis using the TargetScan software and dual-luciferase gene reporter assays indicated that <i>Rela</i> and <i>Traf3</i> were both targeted by miR-155-5p. Meanwhile, the expression of <i>Ptgs2</i> also decreased after transfection of the AR42J cells with miR-155-5p mimic. The opposite results were found when miR-155-5p inhibitor was transfected into the AR42J cells. In addition, we treated caerulein- and TLC-S-induced AR42J cells with the <i>Rela</i> inhibitor helenalin and found that the expression of <i>Rela, Traf3</i> and <i>Ptgs2</i> decreased compared with the NC, while the expression of miR-155-5p did not show any significant difference. Furthermore, we found that miR-155-5p was significantly down-regulated in pancreatitis patients. <b><i>Conclusion:</i></b> miR-155-5p inversely regulated AP development through the Rela/Traf3/Ptgs2 signaling pathway.



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