Supplementary Material for: The <b><i>Drosophila</i></b> Deubiquitinating Enzyme dUSP36 Acts in the Hemocytes for Tolerance to <b><i>Listeria monocytogenes</i></b> Infections
2014-04-23T00:00:00Z (GMT) by
<i>Listeria monocytogenes</i> is a facultative intracellular pathogen which can infect<i> Drosophila melanogaster</i>. Upon infection, <i>Drosophila</i> mounts an immune response including antimicrobial peptide production and autophagy activation. A set of previously published results prompted us to study the role of the deubiquitinating enzyme dUSP36 in response to <i>L. monocytogenes</i> infections. We show in this report that flies with <i>dUsp36</i>-specific inactivation in hemocytes are susceptible to <i>L. monocytogenes</i> infections (as are flies with autophagy-deficient hemocytes) but are still able to control bacterial growth. Interestingly, flies with <i>dUsp36</i>-depleted hemocytes are not sensitized to infection by other pathogens. We conclude that <i>dUsp36</i> plays a major role in hemocytes for tolerance to <i>L. monocytogenes</i>.