000360225_sm_Figures.pdf (769.62 kB)
Supplementary Material for: Non-Axial View of the Varicella-Zoster Virus Portal Protein Reveals Conserved Crown, Wing and Clip Architecture
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posted on 2014-03-14, 00:00 authored by Visalli R.J., Howard A.J.Background: Herpesviridae encode a family of protein homologues that function as the ‘port of entry' for insertion of the viral DNA into preformed capsids during encapsidation. Methods: Transmission electron microscopy (TEM) of recombinant varicella-zoster virus pORF54 was performed. Results: Results suggest that pORF54 forms higher-order structures with itself. Enriched fractions analyzed by TEM revealed non-axial oriented portals with defined central channels and distinguishable crown, wing and clip regions. Conclusion: These morphological features are consistent with those previously reported for other herpesvirus and bacteriophage portal proteins.