Supplementary Material for: M235T Polymorphism in the <b><i>AGT</i></b> Gene and A/G<sup>I8-83</sup> Substitution in the <b><i>REN</i></b> Gene Correlate with End-Stage Renal Disease

<b><i>Background/Aims:</i></b> This study aimed at investigating if M235T polymorphism in the AGT gene and A/G<sup>I8-83</sup> polymorphism in the REN gene correlate with end-stage renal disease (ESRD). <b><i>Methods:</i></b> We analyzed 173 ESRD patients and 329 individuals with normal kidney function for differences in the genotype distribution of AGT-M235T and REN-A/G<sup>I8-83</sup> polymorphisms between the two groups. The data for cases and controls were compared using the χ<sup>2</sup> test. <b><i>Results:</i></b> We found significantly higher levels of serum creatinine and CRP in cases in comparison to controls (p < 0.0001). Data comparison showed a significant association of AGT M235T substitution with ESRD in the dominant model (p = 0.008) and in the comparison of the heterozygous substitution with the homozygous common genotype (p = 0.005). Similarly, REN A/G<sup>I8-83</sup> polymorphism showed a significant difference in the distribution of genotypes between cases and controls (p < 0.038) such that a heterozygous substitution was significantly more common in the ESRD cases in comparison to the homozygous common genotype (p = 0.023). <b><i>Conclusion:</i></b> We conclude that heterozygous substitutions at the AGT M235T and REN A/G<sup>I8-83</sup> loci correlate significantly with ESRD in a north Indian population.