Supplementary Material for: Evolutionary Break Point Analysis between the Proximal Half of Bovine Chromosome 27 and Conserved Segments of the Human Genome

The proximal half of <i>Bos taurus</i> chromosome 27 (BTA27<sub>prox</sub>) delimited by microsatellite markers <i>BM3507</i> and <i>CSSM043</i> reveals complex rearrangements compared to its corresponding <i>Homo sapiens</i> chromosome (HSA) fragments. A comparative mapping approach combining somatic and radiation hybrid cell mapping techniques and related cytogenetic data resulted in an improved physical map for BTA27<sub>prox</sub>, which provides candidate genes for several important economic traits. The generated comprehensive map includes anchor loci for 103 genes and microsatellite markers. Mapping of genes proximal to <i>BM3507</i> matching a region from 0.60 to 2.78 megabase pairs (Mb) of HSA8 confirmed recent sequence annotations on BTA27. Assignments of loci predicted to be on BTA27 to BTA1, BTA8, and BTA17 narrowed down evolutionary chromosome break points compared with corresponding chromosome segments in human. New physical anchors obtained in this study confirm in more detail the described evolutionary conservation between the proximal half of BTA27 and homologous segments of HSA4 and HSA8 and will contribute to the completion of the cattle DNA genome sequence.