Supplementary Material for: Effect of Fluoride-Containing Toothpastes on Enamel Demineralization and Streptococcus mutans Biofilm Architecture

<p>This study aimed to explore the effect of fluoridated toothpastes on biofilm architecture and enamel demineralization in an in vitro biofilm model. <i>Streptococcus mutans</i> was grown on enamel and treated with slurries of commercial toothpastes, containing SnF<sub>2</sub> or NaF. Water and chlorhexidine were used as negative and positive controls, respectively. The developed biofilms were imaged and enamel demineralization was measured. SnF<sub>2</sub> and NaF toothpaste treatments significantly reduced enamel demineralization, but SnF<sub>2</sub> toothpaste was more effective. Only SnF<sub>2</sub> toothpaste and chlorhexidine treatments caused reductions on biofilm mass and thickness. In conclusion, this biofilm model was able to differentiate the effects of the SnF<sub>2</sub> and NaF toothpastes on biofilm architecture and enamel demineralization.</p>