Supplementary Material for: Chromatin Flow Cytometry Identifies Changes in Epigenetic Cell States

2009-09-18T00:00:00Z (GMT) by Obier N. Müller A.M.
We report flow cytometric protocols that quantitatively display the levels of different histone modifications on the single cell level. This method allows the rapid and simultaneous analysis of the global levels of chromatin marks in combination with other flow cytometric features. We show that chromatin flow cytometry identifies changes in epigenetic marks, such as histone acetylation and methylation, in drug-treated and differentiated mouse embryonic stem cell populations.