Supplementary Material for: Characteristic <b><i>miR-24</i></b> Expression in Gastric Cancers among Atomic Bomb Survivors

<b><i>Objective:</i></b> To elucidate the mechanism of radiation-induced cancers, we analyzed the expression profiles of microRNAs extracted from formalin-fixed paraffin-embedded (FFPE) gastric cancer (GC) tissue samples from atomic bomb survivors. <b><i>Methods:</i></b> The expression levels of <i>miR-21</i>, <i>miR-24</i>, <i>miR-34a</i>, <i>miR-106a</i>, <i>miR-143</i>, and <i>miR-145</i> were measured by quantitative reverse transcription polymerase chain reaction (qRT-PCR). <b><i>Results:</i></b> The expression of microRNAs was measured by qRT-PCR in a Hiroshima University Hospital cohort comprising 32 patients in the high-dose-exposed group and 18 patients in the low-dose-exposed group who developed GC after the bombing. The GC cases showing high expression of <i>miR-24</i>, <i>miR-143</i>, and <i>miR-145</i> were more frequently found in the high-dose-exposed group than in the low-dose-exposed group. We next performed qRT-PCR of <i>miR-24</i>, <i>miR-143</i>, and <i>miR-145</i> in a cohort from the Hiroshima Red Cross Hospital and Atomic-Bomb Survivors Hospital comprising 122 patients in the high-dose-exposed group and 48 patients in the low-dose-exposed group who developed GC after the bombing. High expressions of <i>miR-24</i> and <i>miR-143</i> were more frequently found in the high-dose-exposed group than in the low-dose-exposed group. Multivariate analysis demonstrated that only high expression of <i>miR-24</i> was an independent predictor for the exposure status. <b><i>Conclusion:</i></b> These results suggest that the measurement of <i>miR-24</i> expression from FFPE samples is useful to identify radiation-associated GC.