Supplementary Information accompanying proteomics manuscript

These data files accompany the manuscript titled "From shell deposition to protein expression: An integrative assessment of ocean acidification impacts on a marine invertebrate".

Figure S1.  Representative indents made during micromechanical testing for the (A) 400 µatm and (B) 2800 µatm treatment.

Figure S2.  Glycogen content (µg glycogen per mg tissue) for oysters from the pCO2 treatments of 400, 800, and 2800 µatm. There is no difference in glycogen content among treatment groups.

Figure S3.  Heat maps of differentially expressed proteins annotated with protein names. Protein expression values have been log-transformed.

Figure S4.  Representation of key metabolic pathways that are significantly affected by ocean acidification (A), mechanical stress at low pCO2 (B), and mechanical stress at high pCO2 (C).  This includes input files and instructions for viewing data in iPath2.

Table S1.  Raw and normalized (proportion) fatty acid data for 8 oysters each from 3 treatments: 400, 800, and 2800 µatm.

Table S2.  ProteinProphet output for each technical replicate.

Table S3.  Protein expression values (NSAF) for each oyster for the 1 616 proteins identified.

Table S4.  C. gigas proteins with associated SwissProt/UniProt-KB, Gene Ontology (GO), and GO Slim annotations.

Table S5.  Enriched biological processes for proteins >2-fold differentially expressed in the stress responses to elevated pCO2 of 2800 µatm (“OA”), mechanical stress after a one month exposure to 400 µatm (“Mech Stress 400 µatm”), and mechanical stress after a one month exposure to 2800 µtam (“Mech Stress 2800 µatm”).