Supplementary Figure 1 from The stentable in vitro artery: an instrumented platform for endovascular device development and optimization

Quantification of endothelial wound healing. (a) Wound healing is easily visualized by the temporal progression of mean fluorescent intensity across the wound. Positive radial position is toward the bottom of the image as seen in Figure 3b. For clarity, intensity profiles are smoothed and only 1 in 2 time points is shown. (b) The wound boundary was robustly estimated using intensity thresholds varying from 0.1 to 0.5 (identified with symbols). Here, the bottom wound front is shown. For clarity, intensity profiles are smoothed and only 1 in 2 time points is shown. (c) The rate of wound healing is estimated as the slope of the wound front position estimated from each intensity threshold (same symbols as in panel (b)) for the first 7.5-10 hours post-stenting. Despite the wide range of thresholds used, the slope does not change significantly, particularly for early time points in which the wound boundary is most clearly defined.