Supplemental Material for Magwanga et al., 2018

<p><b>Supplementary </b><b>Fig. S1</b>: Phylogenetic tree, gene structure and motif compositions of <i>LEA2</i> genes in upland cotton. The phylogenetic tree was constructed using MEGA 7.0. Exon/intron structures of <i>LEA </i>genes in upland cotton, exons introns and up/down-stream were represented by yellow boxes, black lines and blue boxes, respectively. Protein motif analysis represented by different colours, and each motif represented by number</p><p><b>Supplementary </b><b>Fig. </b><b>S2</b>: conserved motifs in upland cotton, <i>G. hirsutum</i> LEA2 proteins. The conserved motifs were obtained using MEME program. The color scheme of the logo indicates amino acid types. Polar: green=uncharged; blue=+vely charged; red=-vely charged; Non-polar: violet/purple=aliphatic. As described by Dure, 2001; <b>Supplementary </b><b>Fig. </b><b>S3</b>: Alignment of the LEA2 protein sequences of Upland cotton, <i>Gossypium hirsutum</i>. Amino acid sequences were aligned using the ClustalW2 algorithm. Dashes indicate gaps introduced for optimal alignment. The typical LEA2 sequence elements are bound in boxes in different colours: K segment–red box; Y segment–green; S segment–black; E segment–gray; R segment–purple; D segment–yellow</p><p><b>Supplementary </b><b>Fig. </b><b>S4</b>. <i>LEA2 </i>genes distribution in A and D cotton chromosomes: Chromosomal position of each <i>LEA</i>2 genes was mapped according to the upland cotton genome. The chromosome number is indicated at the top of each chromosome</p><p><b>Supplementary </b><b>Fig. </b><b>S5</b>: <i>LEA2</i> genes distribution in upland cotton chromosomes. Chromosomal position of each<i> LEA</i>2 genes was mapped according to the upland cotton genome. The chromosome number is indicated at the top of each chromosome</p><p><b>Supplementary Table S1</b>: List of primers used for upland cotton, <i>G. hirsutum</i> <i>LEA2</i> genes expression analysis under drought stress</p><p><b>Supplementary Table S2</b>: Conserved domain analysis of LEA2s proteins identified from upland cotton using CDD tool from NCBI</p><p><b>Supplementary Table S3</b>: Transmembrane domains in LEA2s identified from upland cotton, using TMHMM and SOSUI servers. ExpAA: The expected number of amino acids in transmembrane helices. First60: The expected number of amino acids in transmembrane helices in the first 60 amino acids of the protein. MP: Membrane protein; TMH: The number of predicted transmembrane helices; <b>Supplementary Table S4</b>: Subcellular location as determined by TargetP and Pprowler prediction tools. SP-secretory pathways; C-chloroplast;-any other location;<b> Supplementary</b> <b>Table S5</b>: Cis element analysis of putative LEA 2 promoters related to drought stress </p><p><b>Supplementary Table S6</b>: <i>LEA2</i> genes and miRNA targets</p>