Supplemental Figure 4. In cells depleted of CD147, collapsed L. monocytogenes membrane protrusions still contain ezrin while comet/rocket tails remain morphologically normal.

(A) HeLa cells were treated with non-targeting control (Ctrl) or CD147-targeted (knockdown [KD]) siRNA sequences, and whole-cell lysates were collected and probed for endogenous CD147 using mouse monoclonal CD147-targeting antibodies. Loading control with α-tubulin is shown below.

(B) Ezrin immunolocalization (green) at actin-rich L. monocytogenes membrane protrusions (red) generated in cells treated with non-targeting control (top) or CD147-targeting (bottom) siRNA sequences. Ezrin is present within the core of wild-type as well as collapsed L. monocytogenes membrane protrusions. Open arrowheads indicate the location of L. monocytogenes bacteria (blue) within their respective membrane protrusion. Scale bar is 10 μm.

(C) Cytosolic comet/rocket tails (solid arrowheads) appear morphologically normal in cells treated with CD147-targeting siRNA sequences. Open arrowheads indicate collapsed and/or contorted L. monocytogenes membrane protrusions. Scale bar is 10 μm.