Subcutaneous injections of IL-2 expand peripheral CD4<sup>+</sup>CD25<sup>+</sup>CD127<sup>low</sup>Foxp3<sup>+</sup> naïve and total memory Tregs.

<p>(A) Gating strategy for naïve CD4<sup>+</sup> CD45RO<sup>-</sup> CD25<sup>+</sup> CD127<sup>low</sup> FoxP3<sup>+</sup>, total memory CD4<sup>+</sup> CD45RO<sup>+</sup> CD25<sup>+</sup> CD127<sup>low</sup> FoxP3<sup>+</sup> and memory CD4<sup>+</sup> CD45RO<sup>+</sup> CD25<sup>+</sup> CD127<sup>low</sup> FoxP3<sup>+</sup> CD39<sup>+</sup>. (B) Proportions of total CD4<sup>+</sup>CD25<sup>+</sup>CD127<sup>low</sup>Foxp3<sup>+</sup> Tregs, (C) naïve CD45RO<sup>-</sup>CD4<sup>+</sup>CD25<sup>+</sup>CD127<sup>low</sup>Foxp3<sup>+</sup> Tregs, (D) total memory CD45RO<sup>+</sup>CD4<sup>+</sup>CD25<sup>+</sup>CD127<sup>low</sup>Foxp3<sup>+</sup> Tregs and (E) memory CD39<sup>+</sup>Tregs, were measured in eighteen patients at wk0, wk16 and wk36 after vaccination and IL-2 injections. Each patient can be distinguished by a colored symbol in the figures. (F) Pie chart showing comparison for compartments of Tregs subsets expansion at wk0, wk16 and wk36. Prism 5.0, version 5.0d, (GraphPad Software, Inc.) was used for statistical analyses. P values were considered significant when < 0.05.</p>