Fig 2.tif (882.37 kB)
Study of cationic lipopolymer/DNA interaction.
figure
posted on 2017-10-11, 17:50 authored by C. Facundo Temprana, M. Jimena Prieto, Daniela E. Igartúa, A. Lis Femia, M. Silvia Amor, Silvia del Valle AlonsoGel retardation assay for (a) DMPC:DC8,9PC:DOTAP (1:1:0.2), DMPC:DC8,9PC:SA (1:1:0.2), DMPC:DC8,9PC:MCL (1:1:0.2) and DMPC:DC8,9PC (1:1) mixtures incubated at 0:1, 6:1, 8:1, 10:1, 12:1, 14:1, 16:1 cationic lipopolymer or lipopolymer/pCH110 plasmid DNA ratios (mol of lipids: mol of base pairs) and (b) DMPC:DC8,9PC:DOTAP (1:1:0.2), DMPC:DC8,9PC:SA (1:1:0.2), DMPC:DC8,9PC:MCL (1:1:0.2) and DMPC:DC8,9PC (1:1) mixtures incubated at 0:1, 8:1, 16:1, 24:1, 30:1, 36:1, 42:1 cationic lipopolymer or lipopolymer/pDsRed plasmid DNA ratios (mol of lipids: mol of base pairs). All lanes were loaded with 1 μg of plasmid DNA.
