Structural and functional analysis of the carotenoid biosynthesis genes of a <i>Pseudomonas</i> strain isolated from the excrement of Autumn Darter

<p>There are many reports about carotenoid-producing bacteria and carotenoid biosynthesis genes. In databases for <i>Pseudomonas</i> genome sequences, there are genes homologous to carotenoid biosynthesis genes, but the function of these genes in <i>Pseudomonas</i> has not been elucidated. In this study, we cloned the carotenoid biosynthesis genes from a <i>Pseudomonas</i> sp. strain, named Akiakane, which was isolated from the excrement of the Autumn Darter dragonfly. Using an <i>Escherichia coli</i> functional expression system, we confirmed that the <i>idi</i>, <i>crtE</i>, <i>crtB</i>, <i>crtI</i>, and <i>crtY</i> gene products of the Akiakane strain show predictable catalytic activities. A cluster of six genes was also found, which was comparable to other carotenoid-producing bacteria that belong to the α-Proteobacteria or γ-Proteobacteria class.</p> <p>The carotenoid biosynthesis genes of a <i>Pseudomonas</i> sp. atrain Akiakane isolated from the excrement of the Autumn Darter, were isolated and analyzed.</p>