Fig 1.TIF (1 MB)
Specificity of the X2F6 mAb.
figure
posted on 2016-10-19, 17:52 authored by Yoshie Kametani, Shino Ohshima, Asuka Miyamoto, Atsuko Shigenari, Masaki Takasu, Noriaki Imaeda, Tatsuya Matsubara, Masafumi Tanaka, Takashi Shiina, Hiroshi Kamiguchi, Ryuji Suzuki, Hitoshi Kitagawa, Jerzy K. Kulski, Noriaki Hirayama, Hidetoshi Inoko, Asako Ando(A) SLA-1*0401, SLA-2*0901, and SLA-3*0602, which are the classical class-I SLA alleles of Haplotype Hp-16.0, and SLA-6*0101, which is a non-classical class-I SLA allele of Hp-16.0, were transfected into HEK293 parent cells, and the reactivity of X2F6 (right panels) was examined by flow cytometry (FCM). Propidium iodide (PI) positive-dead cells were avoided for the gating. PT-85A, the pan-specific MHC class-1 antibody, was used for the positive control (middle panels). (B) The species specificity was examined using swine (Hp-16.0), human, and common marmoset PBMCs. Lymphoid gate was used for the analysis. The percentages shown above the panels are the double-positive cell percentages.
