Spatial variation in cytosolic metabolite concertation and force dynamics under normoxia and hypoxia.

(A): Steady state profiles of ADP/ATP ratio. ADP/ATP is significantly higher in the anoxic cell core under low O2 supply (B): ATP hydrolysis rate is similarly lower in the cell core under hypoxic condition (C): Peak twitch force (Fpeak) is significantly higher in the cell core under hypoxia (D): Increased Fpeak is complemented by increased twitch duration in the cell core, which can lead to loss of energy in the form of shear strain.