ac202559w_si_001.pdf (632.03 kB)
Sol–Gel Derived Nanoporous Compositions for Entrapping Small Molecules and Their Outlook toward Aptamer Screening
journal contribution
posted on 2012-03-20, 00:00 authored by Ji-Young Ahn, SangWook Lee, Minjoung Jo, Jeehye Kang, Eunkyung Kim, Ok Chan Jeong, Thomas Laurell, Soyoun KimThis paper reports for the first time the application
of sol–gel
microarrays for immobilizing nonsoluble small chemicals (Bisphenol-A;
BPA). Also, known problems of sol–gel adhesion to conventional
microtiter well plate substrates are circumvented by anchoring the
sol–gel microspots to a porous silion surface so-called, PS-SG
chips. We confirmed low molecular weight chemical immobilization inside
a sol–gel network using fluorescein. BPA and the BPA specific
aptamer were utilized as a model pair to verify the affinity specific
interaction in the PS-SG selection system. The aptamer interacted
specifically with BPA in the sol–gel spots, as shown in microarrays
forming the letters “L”, “U”, “N”,
and “D”. Moreover, the bound aptamer was released by
heat, recovered, and verified by gel electrophoresis. The developed
PS-SG chip platform will be used for screening aptamers against numerous
small molecules such as toxins, metabolites, or pesticide residues.