Size Matters: Influence of the Size of Nanoparticles on Their Interactions with Ligands Immobilized on the Solid Surface
2010-03-16T00:00:00Z (GMT) by
The correlation between the size of biotinylated nanoparticles and their affinity in relation to interactions with the solid surface was investigated. The silica particles with a diameter of 50−200 nm containing amino groups on the surface were labeled with different quantities of biotin. The affinity properties of biotinylated nanoparticles were studied using a Biacore 3000 instrument equipped with a streptavidin-coated sensor chip (SA chip). It was shown that the increase in the particle size from 50 to 200 nm reduced the affinity (KD) of biotin−streptavidin interactions from 1.2 × 10−12 to 1.2 × 10−10 M. It was found that the particles with higher concentrations of immobilized biotin on particle surfaces demonstrated stronger binding with streptavidin.