Size-exclusion chromatographic analysis of wild-type and S326C OGG1

Copyright information:

Taken from "Dimerization and opposite base-dependent catalytic impairment of polymorphic S326C OGG1 glycosylase"

Nucleic Acids Research 2006;34(5):1620-1632.

Published online 20 Mar 2006

PMCID:PMC1405821.

© The Author 2006. Published by Oxford University Press. All rights reserved

() Non-denatured protein size markers (Sigma). Peak 1, BSA dimer (132 kDa); peak 2, BSA monomer (66 kDa) and peak 3, carbonic anydrase (29 kDa). Purified wild-type OGG1 (100 µg) was analyzed on a Superdex 200 HR column equilibrated with 20 mM Tris–HCl (pH 7.4), 300 mM NaCl at a flow rate of 0.25 ml/min (). Identical runs were performed with 100 µg polymorphic S326C OGG1 () or 100 µg of both wild-type and S326C OGG1 together ().