ac6b02522_si_001.pdf (359.21 kB)
Simultaneous Determination of Protein Kinase A and Casein Kinase II by Dual-Color Peptide Biomineralized Metal Nanoclusters
journal contribution
posted on 2016-10-25, 00:00 authored by Li Zhang, Wei Song, Ru-Ping Liang, Jian-Ding QiuWe
design two artificial substrate peptides to synthesize blue-emissive
Cu nanoclusters and red-emissive Au nanoclusters, respectively. In
addition to the biomineralization function, these two peptides retain
the biological activity to be phosphorylated by protein kinase and
digested by carboxypeptidase Y. In the absence of protein kinase,
the peptides capped on the nanoclusters suffer consecutive exocleavage
by carboxypeptidase Y, resulting in oxidation and thus fluorescence
quenching of the nanoclusters due to the losing of peptide protection.
In the presence of protein kinase A and casein kinase II, the phosphorylation
modification on corresponding substrate peptides protects the peptides
against carboxypeptidase Y digestion and then the fluorescence of
the nanoclusters can be retained. Since a single excitation wavelength
can excite the both nanoclusters, blue and red emissive signals can
be collected at the same time and then the quantitative determination
of protein kinase A and casein kinase II can be achieved simultaneously.