Siglec-1 colocalizes with p24 in VCCs of HIV-1-infected MDMs.
2017-01-27T04:30:02Z (GMT) by
<p><b>(A)</b> MDMs were infected with a biological stock of HIV-1 BaL at a TCID<sub>50</sub> of 0.5/cell. 10 days post-infection cells were fixed and immunolabeled for HIV-1 p24 (green), CD9 (red), tetherin (magenta) and DAPI co-stained. <b>(B)</b> Same immunostaining profile as (A) with the exception of Siglec-1 (red) in place of CD9. Size bar = 10 μm. <b>(C)</b> MDMs were infected with VSV-G pseudotyped NL4.3 and NLUdel <b>(D)</b> at a TCID50 of 2.0/cell. 10 days post-infection cells were fixed and immunostained as described in <b>(B)</b>. All size bars = 10 μm. Representative maximum intensity deconvolved images shown.</p>