Sesquiterpene Glycosides from <i>Cosmospora joca</i>

Five novel sesquiterpene glycosides, namely, cosmosporasides A–E (<b>1</b>–<b>5</b>), were isolated from the ethyl acetate extract of the fermented broth of <i>Cosmospora joca</i>. The structures of <b>1</b>–<b>5</b> were elucidated on the basis of spectroscopic data analyses, monosaccharide composition analyses, and monosaccharide chirality analyses. The relative configuration of the sesquiterpene moiety of <b>3</b>–<b>5</b> was determined by <i>J</i>-based configuration analyses and supported by NOESY assignments. The inhibitory effects of <b>1</b>–<b>5</b> on the nitric oxide (NO) production in lipopolysaccharide (LPS)-activated murine macrophage RAW264.7 cells were evaluated; all except <b>3</b> inhibited NO production at 100 μM. <b>4</b> was the most potent, with an average maximum inhibition and a median inhibitory concentration value of 71.70 ± 1.23% and 65.98 ± 0.53 μM, respectively.