Sesquiterpene Glycosides from Cosmospora joca

Five novel sesquiterpene glycosides, namely, cosmosporasides A–E (1–5), were isolated from the ethyl acetate extract of the fermented broth of Cosmospora joca. The structures of 1–5 were elucidated on the basis of spectroscopic data analyses, monosaccharide composition analyses, and monosaccharide chirality analyses. The relative configuration of the sesquiterpene moiety of 3–5 was determined by J-based configuration analyses and supported by NOESY assignments. The inhibitory effects of 1–5 on the nitric oxide (NO) production in lipopolysaccharide (LPS)-activated murine macrophage RAW264.7 cells were evaluated; all except 3 inhibited NO production at 100 μM. 4 was the most potent, with an average maximum inhibition and a median inhibitory concentration value of 71.70 ± 1.23% and 65.98 ± 0.53 μM, respectively.