Fig 5.TIF (1.13 MB)

Sema6A cytoplasmic domain interacts with Abl and Mena.

figure

posted on 2016-07-08, 16:32 authored by Francesc Perez-Branguli, Yvrick Zagar, Daniel K. Shanley, Isabella A. Graef, Alain Chédotal, Kevin J. Mitchell

(A) Immunoprecipitations from untreated or PlxnA2-EC-treated COS-7 cells previously transfected with different combinations of myc-Sema6A, Abl-V5 and PlxnA2. Immunoprecipitations (Ip) were performed employing an anti-V5 antibody. Antibodies against V5, myc and PlxnA2 were used in the immunoblots (Ib). (B) Immunoprecipitations from untreated or PlxnA2-EC treated COS-7 cells previously transfected with different combinations of myc-Sema6A, Mena-V5 and PlxnA2. Immunoprecipitations (Ip) were performed employing an anti-myc antibody. Antibodies against V5, myc and PlxnA2 were used in the immunoblots (Ib). (C) and (D) Interactions were quantified by immunoblotting and densitometric analysis. Histograms represent quantification of interaction normalized to the amount of immunoprecipitated protein (Abelson-V5 for Sema6A-Abelson or Sema6A-myc for Sema6A-Mena) from three independent experiments. Students t-test *p<0,05 indicating signifiance. S6A-Abl/S6A-Abl+PlA2 p = 0.013, S6A-Abl-PlA2/S6A-Abl-PlA2+PlA2 p = 0.034. Errors bars represent standard error.