ja071774j_si_001.pdf (787.48 kB)
Self-Assembly Dynamics of a Cylindrical Capsule Monitored by Fluorescence Resonance Energy Transfer
journal contribution
posted on 2007-07-18, 00:00 authored by Elizabeth S. Barrett, Trevor J. Dale, Julius RebekThe constituent cavitands of a cylindrical capsule were labeled with donor and acceptor
fluorophores, and fluorescence resonance energy transfer (FRET) was employed as a tool to study the
dynamics of self-assembly. When donor and acceptor dyes are present in the same capsular assembly,
they are brought within 25 Å of each other, a distance suitable for efficient energy transfer to occur between
them. This allowed for the study of interacting species at nanomolar concentrations providing information
unattainable from NMR experiments. The kinetic stability of the capsule in the presence of various guest
molecules was investigated which revealed a range of more than 4 orders of magnitude in the rates of
cylindrical capsule exchange. While the thermodynamic stability of the capsule generally dictates the self-assembly dynamics, it was discovered that longer rigid guests can impart a significant kinetic barrier to
monomer exchange.
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fluorescence resonance energy transferdynamicstabilityacceptor fluorophores25 Åcapsule exchangedonorFRETnanomolar concentrationsCylindrical Capsule Monitoredcapsular assemblyFluorescence Resonance Energy TransferThe constituent cavitandsacceptor dyesNMR experimentsmonomer exchange4 ordersguest moleculesenergy transfer
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