am7b01719_si_001.pdf (670.03 kB)
Self-Etch Adhesive as a Carrier for ACP Nanoprecursors to Deliver Biomimetic Remineralization
journal contribution
posted on 2017-05-19, 14:19 authored by Zhifang Wu, Xiaokan Wang, Zhe Wang, Changyu Shao, Xiaoting Jin, Leiqing Zhang, Haihua Pan, Ruikang Tang, Baiping FuLab biomineralization
should be carried out in an actual clinical practice. This study evaluated
self-etch adhesive as a carrier for amorphous calcium phosphate (ACP)
nanoprecursors to continuously deliver biomimetic remineralization
of self-assembly type I collagen and demineralized dentin. Si-containing
ACP particles (Si-ACP) stabilized with polyaspartic acid (PAsp) were
synthesized and characterized by transmission electron microscopy
(TEM), scanning electron microscopy–energy-dispersive X-ray
spectroscopy, Fourier transform infrared analysis, X-ray powder diffractometry,
and X-ray phototelectron spectroscopy. The biomimetic remineralization
of single-layer reconstituted type I collagen fibrils and demineralized
dentin was analyzed by using two one-bottle self-etch dentin adhesives
(Clearfil S3 Bond (S3), Kurraray-Noritake; Adper Easy One (AEO), 3
M ESPE) as a carrier loaded (or not, in the case of the control) with
25 wt % of Si-ACP particles. In vitro cytotoxicity assessed by the
Cell Counting Kit-8 indicated that the Si-ACP particles had no adverse
effect on cell viability. The capacity for Ca and P ions release from
cured Si-ACP-containing adhesives (S3, AEO) was evaluated by inductively
coupled plasma-atomic emission spectrometry, revealing the successively
increasing release of Ca and P ions for 28 days. The intra- and extrafibrillar
remineralization of type I collagen and demineralized dentin was confirmed
by TEM and selected-area electron diffraction when the adhesives were
used as a carrier loaded with Si-ACP particles. Therefore, we propose
self-etch adhesive as a novel carrier for ACP nanoprecursors to continuously
deliver biomimetic remineralization.
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ESPEClearfil S 3 BondSi-containing ACP particlesplasma-atomic emission spectrometryX-ray phototelectron spectroscopynanoprecursorAEOdemineralized dentincarriercollagenTEMone-bottle self-etch dentin adhesivesSi-ACP particlestransmission electron microscopyBiomimetic Remineralization Lab biomineralizationsingle-layer reconstituted typeselected-area electron diffractionbiomimetic remineralizationP ions releaseX-ray powder diffractometry
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