Secondary metabolites from <i>Triclisia gilletii</i> (De Wild) Staner (Menispermaceae) with antimycobacterial activity against <i>Mycobacterium tuberculosis</i>

<p>Triclisinone (<b>2</b>), a new ochnaflavone derivative, was isolated from the aerial parts of <i>Triclisia gilletii</i>, along with known drypemolundein B (<b>1</b>) and eight other known compounds. The chemical shifts of drypemolundein B (<b>1</b>) have been partially revised based on reinterpretation of NMR spectroscopic data. The eight other secondary metabolites are composed of: (+)-nonacosan-10-ol (<b>3</b>); stigmasterol (<b>4</b>), 3-<i>O</i>-β-D-glucopyranosylsitosterol (<b>5</b>), 3-<i>O</i>-β-D-glucopyranosylstigmasterol (<b>6</b>); oleanic acid (<b>7</b>); myricetin (<b>8</b>), quercetin (<b>9</b>) and 3-methoxyquercetin (<b>10</b>). Their structures were elucidated using IR, MS, NMR 1D and 2D, <sup>1</sup>H and <sup>13</sup>C and comparison with literature data. Furthermore, compounds <b>1</b>, <b>2</b>, <b>5, 6, 8, 9</b> and the crude extract were tested against <i>Mycobacterium tuberculosis</i>. Compounds <b>1, 2, 8</b> and <b>9</b> displayed moderate to very good activity against resistant strain (codified AC 45) of <i>M. tuberculosis</i> with minimum inhibitory concentrations MICs ranging from 3.90 to 62.5 μg/mL<i>.</i></p>