SUMO conjugates accumulate during productive EBV infection.

Akata-Bx1 cells were harvested at the indicated times after induction by anti-IgG cross-linking and western blots were probed with antibodies to SUMO1 or SUMO2/3. (A) Induction of the productive cycle was confirmed in western blots probes with antibodies to the EBV transactivator BZLF1. Representative western blots illustrating the progressive accumulation of high molecular weight polypeptides reacting with antibodies to SUMO1 (B) and SUMO2/3 (C) antibodies; β-actin was used as loading control. * a longer exposure was required to visualize the free SUMO1.