SDS-PAGE analysis of recombinant <i>S</i>. <i>plymuthica</i> AS9 PalI and its variants expressed in <i>E</i>. <i>coli</i> BL21(DE3).

<p>(A) <i>Lane</i> M, molecular weight markers; <i>Lane</i> 1, culture supernatant of the wild-type enzyme; Sucrose isomerase is indicated by the black arrow. (B) <i>Lane</i> M, molecular weight markers; <i>Lane</i> 1, purified wild-type enzyme; <i>Lane</i> 2, purified E175N; <i>Lane</i> 3, purified K576D; <i>Lane</i> 3, purified E175N/K576D.</p>