SDS-PAGE analysis of purified pp-PA83 variants.

<p>Lanes were loaded with 1.75 μg per lane. M: molecular weight markers, A: pp-dPA83 (<i>in vivo</i> deglycosylated), B: glycosylated pp-PA83, C: <i>in vitro</i> deglycosylated pp-PA83, D: pp-PA83M-Q mutant, E: pp-PA83M-D mutant. The arrowhead in lane C indicates migration of PNGase F.</p>