Fig 1.TIF (914.57 kB)
SDS-PAGE analysis of purified pp-PA83 variants.
figure
posted on 2016-04-21, 05:56 authored by Tarlan Mamedov, Jessica A. Chichester, R. Mark Jones, Ananya Ghosh, Megan V. Coffin, Kristina Herschbach, Alexey I. Prokhnevsky, Stephen J. Streatfield, Vidadi YusibovLanes were loaded with 1.75 μg per lane. M: molecular weight markers, A: pp-dPA83 (in vivo deglycosylated), B: glycosylated pp-PA83, C: in vitro deglycosylated pp-PA83, D: pp-PA83M-Q mutant, E: pp-PA83M-D mutant. The arrowhead in lane C indicates migration of PNGase F.
