SDS-PAGE analysis of purified pp-PA83 variants.

Lanes were loaded with 1.75 μg per lane. M: molecular weight markers, A: pp-dPA83 (in vivo deglycosylated), B: glycosylated pp-PA83, C: in vitro deglycosylated pp-PA83, D: pp-PA83M-Q mutant, E: pp-PA83M-D mutant. The arrowhead in lane C indicates migration of PNGase F.