SB203580 treatment reduces the phosphorylation of MAPKAPK2 and HSP27.

Proteins were extracted from the liver tissue samples of 2%DMSO-treated (un-infected), 2%DMSO-treated DENV-infected, and SB203580-treated DENV-infected groups of mice. An additional cocktail of phosphatase inhibitors was added for maintaining the phosphorylated proteins and allowed them to standard Western blot analysis with specific antibodies. The results were shown (A) phosphorylated MAPKAPK2 and total MAPKAPK2 and (C) phosphorylated HSP27 and total HSP27, which is normalized to their respective GAPDH. The results shown are representative of three independent experiments with three mice (n = 3) from each group. A densitometry analysis was conducted with the individual blots normalized to GAPDH and is shown in (B) phosphorylated MAPKAPK2 and total MAPKAPK2 and (D) phosphorylated HSP27 and total HSP27.