SA regulates Pha13 at post-translational level.

(A) Total proteins were extracted from leaves of P. aphrodite without treatment (0 h), treated with H₂O (Mock), or SA at different times (h), and used for in vivo immunoprecipitation (IP) assay using anti-Pha13 antibody. Samples after IP were analyzed by immunoblotting using anti-Pha13 or anti-ubiquitin antibody. (B) P. aphrodite was treated with H₂O (Mock) or SA, and immediately followed by infiltration of DMSO (-) or MG132 (+). Total proteins were extracted from leaves of the treated samples at 3 h post-treatment, and were used for in vivo IP assay using anti-Pha13 antibodies. Samples after IP were analyzed by immunoblotting using anti-Pha13 antibody. (A and B) Extracted total proteins (input) stained by coomassie brilliant blue (CBB) served as a loading control.