Ros1 alters the expression of the effector repertoire late during infection.

All genes encoding secreted effectors without predicted functional domain are listed and their up regulation or downregulation by Ros1 is indicated. Based on the RNA-seq data comparing gene expression in FB1 x FB2 and the corresponding ros1 deletion strains 8 days after infection of maize seedlings and the ChIP-seq data from FB1Δros1-Ros1HA x FB2Δros1-Ros1HA at the same time point, effector genes were sorted in 5 different categories presented as histograms: directly downregulated, indirectly downregulated, directly upregulated, indirectly upregulated and not differentially regulated by Ros1. The percentage (Y axis) represented by each category is calculated relative to the total number of effector genes without functional domains (320 genes based on [47] and secretion prediction by SignalP 4.1 [48]). Colors refer to the fold change of transcripts in wild type compared to ros1 deletion strains in the RNA-seq analysis; the darker the color the higher the fold change. Downregulated effector genes are indicated in blue, upregulated effector genes are indicated in red, effector genes not regulated by Ros1 are indicated in yellow. All genes names or identification numbers are listed; genes which have been characterized or mentioned in previous publications are indicated in bold characters. Leaf-specific effector genes without functional domains [13] showing virulence defects after seedling infection are shown in white, tassel-specific effector genes without functional domains [13] are shown in green. cmu1 [8] and three other differentially regulated effector genes (UMAG_03615 [10], UMAG_11763, UMAG_01130 [13]) are not included in this figure as they contain a functional domain.