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Role of Protein Charge Density on Hepatitis B Virus Capsid Formation
journal contribution
posted on 2018-04-20, 16:03 authored by Xinyu Sun, Dong Li, Zhaoshuai Wang, Panchao Yin, Rundong Hu, Hui Li, Qiao Liu, Yunyi Gao, Baiping Ren, Jie Zheng, Yinan Wei, Tianbo LiuThe role of electrostatic
interactions in the viral capsid assembly
process was studied by comparing the assembly process of a truncated
hepatitis B virus capsid protein Cp149 with its mutant protein D2N/D4N,
which has the same conformational structure but four fewer charges
per dimer. The capsid protein self-assembly was investigated under
a wide range of protein surface charge densities by changing the protein
concentration, buffer pH, and solution ionic strength. Lowering the
protein charge density favored the capsid formation. However, lowering
charge beyond a certain point resulted in capsid aggregation and precipitation.
Interestingly, both the wild-type and D2N/D4N mutant displayed identical
assembly profiles when their charge densities matched each other.
These results indicated that the charge density was optimized by nature
to ensure an efficient and effective capsid proliferation under the
physiological pH and ionic strength.
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assembly profilescapsid protein self-assemblycharge densitieshepatitis B virus capsid protein Cp 149capsid assembly processD 2Nprotein charge densityProtein Charge Densityassembly processcharge densitycapsid proliferationcapsid aggregationcapsid formationbuffer pHprotein D 2Nprotein concentrationHepatitis B Virus Capsid Formationprotein surface charge densities
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